黄羽祖代公鸡精液作为禽白血病净化检测材料的应用研究

doi:10.11843/j.issn.0366-6964.2017.01.015

Abstract

Abstract:

In order to investigate the role of semen of yellow feather grandparent roosters in avian leukosis (AL) eradication process，a, b, c three different breeds of yellow feather grandparent roosters were chosen to be sampled. ALV p27 antigen ELISA was used in direct detection of cloacal swabs, while anti-coagulated blood and semen sample were used to isolate and detect exogenous ALV based on DF-1 cells. Upon comparative analysis, the results showed that the virus could be isolated from semen, while the result of ALV p27 antigen ELISA S/P value of cloacal swabs and the virus isolation of plasma were negative. Three results couldn’t completely correspond respectively. Individual AL deteetion of cloacal swabs, plasma or semen could lead to missed detection. Therefore in AL eradication of yellow feather breeds, semen as the test sample is feasible and should not be neglected, multiple samples should be considered in order to improve the detection rate and speed up the purification process.

CLC Number:

S852.659.3

RAO Ming-zhang, YUAN Li-xia, ZHAO Zi-jun, ZHANG Jie, LI Jin-qun, LIAO Ming, CAO Wei-sheng. Applied Research of Yellow Feather Grandparent Roosters Semen as Test Sample in Avian Leukosis Eradication Program[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 124-131.

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Applied Research of Yellow Feather Grandparent Roosters Semen as Test Sample in Avian Leukosis Eradication Program

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